The Thalassemias

acetate acid activity added addition adult amount analysis anemia associated blood buffer carrier cellulose cellulose acetate cent changes chromatography clinical column concentration condition containing counts described detection determined diagnosis distribution effect electrophoresis elevated elution enzyme fetal Figure filter findings fragments g/dl globin chain globin gene H disease Hb A₂ Hb Bart's Hb F Hb H hematologic hemoglobin heterozygotes heterozygous homozygous human hybridization inclusion increase incubation individual iron Journal laboratory less level of Hb liver loading major maternal measurement method mixed mixture necessary normal obtained patients population possible prepared present probe problem procedure produced protein radioactivity range ratio reaction red cell reduced reference removed restriction samples screening separation serum severe shown shows solution specific stain stored studies synthesis technique thalassemia trait transfusion tube usually values variants volume washed Weatherall