3D video synchrotron x-ray microtomography of Phloeocharis agerata Chatzimanolis Newton and Engel (Coleoptera: Staphylinidae)

"The specimen was imaged at beamline ID19 in the European Synchrotron Radiation Facility (ESRF, Grenoble) using a propagation phase protocol as described in Tafforeau et al. (2006) and Soriano et al. (2010). The scan consisted of 2000 projections acquired through a 180° rotation and 0.3 seconds of exposure time, and a beam set at 20 keV using a 17.6 m period undulator with a gap of 14.5 mm and 2 mm of aluminum as filter. The scan voxel size was 1.4 microns, and the propagation distance 25 mm. After the scan the volume was reconstructed using a back filtered projection algorithm especially adapted for local tomography (PyHST, ESRF), and later segmented using a manual region growing approach in VGStudioMax software" from the materials and methods of the paper linked below.