The purpose of this study was to evaluate the hepatoprotective and anti-fibrotic actions of crude extracts of Ganoderma tsugae (GTE) on chronic liver injury induced by carbon tetrachloride (CCl4) in rats. CCl4 (20%, 0.5 ml/rat) was given twice a week for 8 weeks, and animals received GTE through the whole experimental period. GTE showed obvious reducing actions on the elevated levels of glutamate-oxalate-transaminase (GOT) and glutamate-pyruvate-transaminase (GPT) caused by CCl4 at weeks 3, 6 and 8. Liver fibrosis in rats induced by CCl4 led to the drop of serum albumin and hepatic protein concentrations, while GTE increased serum albumin and hepatic protein concentrations. The CCl4-induced liver fibrosis may prolong the prothrombine time and increase albumin/globulin (A/G) ratio. GTE significantly decreased the prothrombine time and A/G ratio. Liver fibrosis induced by CCl4 markedly increased the weight of the spleen, hepatic water and hydroxyproline contents in rats, while GTE decreased the rat's spleen weights, hepatic water and hydroxyproline contents. All these results clearly demonstrated that GTE has hepatoprotective and anti-fibrotic activities.